Chromatography Essay examples -- essays research papers

Chromatography is a separation technique in which the mixture to be separated is turn in a solvent and the resulting solution, often called the mobile phase, is then passed through or over another material, the stationary phase. The separation of the pilot mixture depends on how strongly each component is attracted to the stationary phase. Substances that are attracted strongly to the stationary phase will be retarded and not fail alone with the mobile phase. Weakly attracted substances will move more rapidly with the mobile phase.Liquid chromatography is an analytical technique that is useful for separating ions or molecules that are dissolved in a liquid phase. If the sample solution is in contact with a second solid or liquid phase, the different solutes will act with the other phase to differing degrees due to differences in adsorption, ionic strength, polarity or size. These differences allow the mixture components to be separated from each other by employ these difference s to determine the transit time of the solutes through a column.Simple liquid chromatography consists of a column with a fritted bottom that holds a stationary phase in equilibrium with a solvent. Typical stationary phases (and their interactions with solutes) are solids (adsorption), ionic groups on a resin (ion-exchange), liquids on an unbiased solid support (partitioning), and porous inert particles (size exclusion). The mixtur...